nebnext multiplex oligos for illumina (New England Biolabs)
Structured Review

Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebnext+ultra+ii+ligation+module/NEBNext+Ultra+II+Ligation+Module/pmc13651819-675-34-34
Average 97 stars, based on 527 article reviews
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1) Product Images from "Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses"
Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses
Journal: iScience
doi: 10.1016/j.isci.2026.117639
Figure Legend Snippet:
Techniques Used: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software
Related Articles
Ligation:Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing Article Snippet: The mix of NTPs was composed of 40 μL of the 10 mM dATP, 4 μL of the 10 mM dCTP, and 4 μL of the 10 mM dGTP solution (N0446S, NEB). .. Finally, we ligated 1 μL of adapter B using Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity Article Snippet: .. Ligation was carried out using the Article Title: Socially regulated genes are spatially hyperconnected to enhancers in the ant brain Article Snippet: .. For end repair and adapter ligation, DNA was first resuspended in a master mix prepared using NEBNext Ultra II DNA Library Prep Kit (3.5 μL reaction buffer, 1.5 μL enzyme mix, 25 μL H 2 O) (NEB, E7645S), and ligated to NEB Illumina universal adapters using components from Article Title: Multi-omics profiling reveals microenvironmental remodeling as a key driver of house dust mite-induced lung cancer progression Article Snippet: .. Libraries were prepared using the NEBNext Ultra II End Repair/dA-Tailing Module (Cat. No E7546L), the Article Title: Tumor-infiltrating T Lymphocytes Recognize Thyroid-specific Proteins and Neo-antigens in Follicular Cell-derived Thyroid Cancers Article Snippet: .. Genomic DNA was fragmented enzymatically using the NEBNext Ultra II FS module, followed by Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. Article Title: High-resolution retrospective single cell lineage tracing with mutable homopolymers Article Snippet: Following lysis, single-cell gDNA was digested with MseI (Thermo Fisher Scientific) and MspI (New England Biolabs) and simultaneously A-tailed using Klenow fragment (exo-) (Thermo Fisher Scientific). .. NEBNext EM-seq adapters (New England Biolabs) were ligated using the Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity. Article Snippet: .. Ligation was carried out using the Incubation:Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity Article Snippet: .. Ligation was carried out using the Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity. Article Snippet: .. Ligation was carried out using the Adapter Ligation:Article Title: Socially regulated genes are spatially hyperconnected to enhancers in the ant brain Article Snippet: .. For end repair and adapter ligation, DNA was first resuspended in a master mix prepared using NEBNext Ultra II DNA Library Prep Kit (3.5 μL reaction buffer, 1.5 μL enzyme mix, 25 μL H 2 O) (NEB, E7645S), and ligated to NEB Illumina universal adapters using components from Binding Assay:Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. Reverse Transcription:Article Title: NAP-seq for full-length noncapped RNA sequencing. Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution. |