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nebnext multiplex oligos for illumina  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext multiplex oligos for illumina

    Nebnext Multiplex Oligos For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+ultra+ii+ligation+module/NEBNext+Ultra+II+Ligation+Module/pmc13651819-675-34-34
    Average 97 stars, based on 527 article reviews
    nebnext multiplex oligos for illumina - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses"

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    Journal: iScience

    doi: 10.1016/j.isci.2026.117639


    Figure Legend Snippet:

    Techniques Used: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

    Related Articles

    Ligation:

    Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing
    Article Snippet: The mix of NTPs was composed of 40 μL of the 10 mM dATP, 4 μL of the 10 mM dCTP, and 4 μL of the 10 mM dGTP solution (N0446S, NEB). .. Finally, we ligated 1 μL of adapter B using NEBNext Ultra II Ligation Module (E7595L, NEB). .. Adapter B was formed by synthesizing and annealing GATCGGAAGAGCACACGTCTGAACTCCAGTC-[3′ Amino Modifier] and GACTGGAGTTCAGACGTGTGCTC TTCCGATC-S-T with methylated C (IDT).

    Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity
    Article Snippet: .. Ligation was carried out using the NEBNext Ultra II Ligation Module (NEB, #7595S) incubated at 20 °C for 30 min followed by 16 °C overnight. .. Dynabeads (Invitrogen, #11205D) were washed twice in 1× Block and Wash (B&W) buffer (prepared as 2× B&W: 10 mM Tris-HCl pH 7.5, 2 M NaCl, 1 mM EDTA) and resuspended in 2× B&W.

    Article Title: Socially regulated genes are spatially hyperconnected to enhancers in the ant brain
    Article Snippet: .. For end repair and adapter ligation, DNA was first resuspended in a master mix prepared using NEBNext Ultra II DNA Library Prep Kit (3.5 μL reaction buffer, 1.5 μL enzyme mix, 25 μL H 2 O) (NEB, E7645S), and ligated to NEB Illumina universal adapters using components from NEBNext Ultra II Ligation Module (15 μL ligation master mix, 0.5 μL ligation enhancer) (NEB, E7595S). .. Afterwards, 1.5 μL NEB USER enzyme (NEB, M5505S) was added and samples were incubated for 15 min at 37°C.

    Article Title: Multi-omics profiling reveals microenvironmental remodeling as a key driver of house dust mite-induced lung cancer progression
    Article Snippet: .. Libraries were prepared using the NEBNext Ultra II End Repair/dA-Tailing Module (Cat. No E7546L), the NEBNext Ultra II Ligation Module (Cat. No E7595L) and indexed using i7 and i5 primers. .. DNA libraries were submitted to Novogene (Sacramento, CA) and sequencing on Illumina NovaSeq X-Plus Sequencing Platform.

    Article Title: Tumor-infiltrating T Lymphocytes Recognize Thyroid-specific Proteins and Neo-antigens in Follicular Cell-derived Thyroid Cancers
    Article Snippet: .. Genomic DNA was fragmented enzymatically using the NEBNext Ultra II FS module, followed by NEBNext Ultra II Ligation module (E7810 and E7959, New England Biolabs) with IDT xGen Stubby Adapters (Integrated DNA Technologies). .. Next, we performed postligation cleanup and 800-bp DNA fragments were isolated by dual size selection with Agencourt AMPure XP beads (Beckman Coulter).

    Article Title: NAP-seq for full-length noncapped RNA sequencing.
    Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution.

    Article Title: High-resolution retrospective single cell lineage tracing with mutable homopolymers
    Article Snippet: Following lysis, single-cell gDNA was digested with MseI (Thermo Fisher Scientific) and MspI (New England Biolabs) and simultaneously A-tailed using Klenow fragment (exo-) (Thermo Fisher Scientific). .. NEBNext EM-seq adapters (New England Biolabs) were ligated using the NEBNext Ultra II Ligation Module (New England Biolabs). .. The library was amplified for 20 cycles with a single primer (Bst_RCA_Primer_v4; Supplementary Table 2) using KAPA HiFi DNA Polymerase (Roche).

    Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity.
    Article Snippet: .. Ligation was carried out using the NEBNext Ultra II Ligation Module (NEB, #7595S) incubated at 20 °C for 30min followed by 16 °C overnight. .. Dynabeads (Invitrogen, #11205D) were washed twice in 1× Block and Wash (B&W) buffer (prepared as 2× B&W: 10mM Tris-HCl pH 7.5, 2M NaCl, 1mM EDTA) and resuspended in 2× B&W.

    Incubation:

    Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity
    Article Snippet: .. Ligation was carried out using the NEBNext Ultra II Ligation Module (NEB, #7595S) incubated at 20 °C for 30 min followed by 16 °C overnight. .. Dynabeads (Invitrogen, #11205D) were washed twice in 1× Block and Wash (B&W) buffer (prepared as 2× B&W: 10 mM Tris-HCl pH 7.5, 2 M NaCl, 1 mM EDTA) and resuspended in 2× B&W.

    Article Title: Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity.
    Article Snippet: .. Ligation was carried out using the NEBNext Ultra II Ligation Module (NEB, #7595S) incubated at 20 °C for 30min followed by 16 °C overnight. .. Dynabeads (Invitrogen, #11205D) were washed twice in 1× Block and Wash (B&W) buffer (prepared as 2× B&W: 10mM Tris-HCl pH 7.5, 2M NaCl, 1mM EDTA) and resuspended in 2× B&W.

    Adapter Ligation:

    Article Title: Socially regulated genes are spatially hyperconnected to enhancers in the ant brain
    Article Snippet: .. For end repair and adapter ligation, DNA was first resuspended in a master mix prepared using NEBNext Ultra II DNA Library Prep Kit (3.5 μL reaction buffer, 1.5 μL enzyme mix, 25 μL H 2 O) (NEB, E7645S), and ligated to NEB Illumina universal adapters using components from NEBNext Ultra II Ligation Module (15 μL ligation master mix, 0.5 μL ligation enhancer) (NEB, E7595S). .. Afterwards, 1.5 μL NEB USER enzyme (NEB, M5505S) was added and samples were incubated for 15 min at 37°C.

    Binding Assay:

    Article Title: NAP-seq for full-length noncapped RNA sequencing.
    Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution.

    Reverse Transcription:

    Article Title: NAP-seq for full-length noncapped RNA sequencing.
    Article Snippet: The majority of the mammalian genome is transcribed into RNAs, most of which are noncapped RNAs (napRNAs) that not only regulate diverse biological processes through their functions as noncoding RNAs but also serve as processing products to delineate specific RNA biogenesis pathways.. However, due to their heterogeneous lengths, diverse terminal modifications and complex secondary structures, identifying these napRNAs poses substantial challenges.. Recently, we developed a napRNA sequencing technique (NAP-seq) to identify full-length sequences of napRNAs with various terminal modifications at single-nucleotide resolution.



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    Image Search Results


    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: Libraries were prepared by blunting and dA-tailing the amplicons using the End Prep library kit (NEB; Ref: E7546) followed by NEBNext Ultra II Ligation Module kit (NEB, Ref: E7595) with the adapters obtained from NEBNext Multiplex Oligos for Illumina (NEB, Ref:E7600) following standard protocols.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software